Incucyte Zoom Imager | Cancer Research Institute | School of Medicine | Queen's University
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Incucyte zoom download.Empower your Research with Incucyte® Live-Cell Analysis
Incucyte Base Software | Sartorius.Software Modules for the IncuCyte® ZOOM System
Page 50 that the software is functioning within the specified parameters. These Metrics will not be relevant to the User under normal circumstances. Median will select the image Mean or image Median respectively for graphing. Page 51 Regions and Grouping Select the Region pull-down menu to display all possible microplate regions. The Region All Wells meaning the entire plate provides the largest number of grouping possibilities. Page 52 When Custom Region is selected, the vessel color will change from light orange to green, thus alerting the User that this is a special graphing Mode.
Normally, when a graph is generated, it is automatically assigned a Label based upon the selected Region and Statistic. Histograms can only be generated for a single time point, so select Single Time instead of Time Range and then select the Histogram radio button.
Page 54 Figure Histogram 7. Left- click twice on the graph background area and the Customization Window will appear as shown in Figure This window can be used to set a variety of graph attributes including graph style, axis, font, etc. Page 55 7. In these cases, the graph can be dragged as either the raw data or the image. Page 57 Outlier Removal This function facilitates the increasingly stringent removal of outlying vessel data. Selecting Outlier Removal will reduce the standard deviation of the Metrics.
The outlying data are selected automatically by the software according to an algorithm - points cannot be excluded manually. If the Calendar Mode box is selected, the x-axis of the graph will be displayed as year, month, day and time.
Page 59 Another useful application of the Auto-Alignment function is the comparison of multiple traces at different stages of growth. Page 60 7. File Use the File Menu to open, save, or print a graph or to close the window.
Edit The Edit Menu provides the option to edit the graph title and labels associated with traces on the graph. Page Microplate Graphing 7. To zoom, left-click the mouse and then highlight the portion of the graph to be expanded. Release the mouse to see the Zoomed portion of the graph.
Page Exporting Experimental Data Figure Example Microplate Graph 7. Export parameters can be adjusted according to the desired Layout, Destination and Other options. Page 63 data sets are desired, then each data set must be exported individually Single Time exports. Page 64 Show each Scan as its own Table Alternatively, the data can be displayed as a single table, one table for each scan time.
If each scan is displayed as its own table, the columns labels can be displayed or not by checking the corresponding check box. Page 65 Include Error Measurements Break Data Into Individual Images Include Experiment Details in the Header Choose this box to include export of the following additional details along with the data: the selected Metric usually confluence , cell type, passage number, and any notes. If multiple files are exported, the additional details will appear in every file.
Page Saving And Printing Graphs 4. Select the desired time from the Time Tree located at the top-left of the screen. Right-click and hold anywhere within the table displaying the assay metrics. Use the mouse to drag it into the target software and release the mouse button. Page 69 Figure Page 71 down menu.
The movie options will still be available in this menu, but if you try to export single images as a movie, you will get an error message. The prefix will be automatically assigned additional time and area information that will appear in the Example box below. Page Exporting Movies Overview 7. To export movies follow the steps indicated below. Page Archiving addition to these customization options, there is also a frames-per- second parameter that can be adjusted from a minimum of 1 to a maximum of 30 frames per second.
When the mouse is placed over this control, a thumbnail preview of the movie at the selected frame rate is shown as an aid. Page Archiving Vessels Important concepts to remember while creating an archive are listed below: Due to the volume of data involved, archives are more likely to be written successfully when the archive size is kept small.
Essen BioScience recommends archiving data on a monthly basis or more frequently. If access is desired, the ZOOM- attached storage must be removed and attached to your local computer. Page 78 Figure When placing trays into the drawer, make sure that the drawer Alignment Posts pass through the tray Alignment Windows see Figure The tray should sit very flat, and the posts should prevent any lateral movement.
Page Changing Objectives 7. Users with administrator privileges are the only users who can change the objectives within the software. Changing objectives is manual, therefore it is suggested that the User organize the workflow such that the objective is changed after a length of time as opposed to changing it on a daily basis.
Page 80 Use a 2. Loosen and rotate the objective counterclockwise to remove it from the mount. Place the new objective on the mount and rotate clockwise. Be sure to rotate the objective until it is tight in the mount. Page Plate Map Editor 7. The wound healing application for the zoom is a little more complex than other applications by virtue of its setup. We have a wound making module for 96 well plates with a specific protocol to be followed.
Fuller details and example of the applications possible on the incucyte zoom can be found at the essen bio web page here,. Microbiological Testing. Moisture Analysis. Lab Weighing. My Request. I would like to sign up for newsletters from Sartorius Sartorius AG and its affiliated companies based of my personal interests. I can revoke my consent at any time with effect for the future by sending an e-mail to unsubscribe sartorius. Request a Demo, Literature, or More Information.
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- ESSEN BIOSCIENCE INCUCYTE ZOOM USER MANUAL Pdf Download | ManualsLib
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